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KMID : 0380020030180010035
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Korean Journal of Biotechnology and Bioengineering
2003 Volume.18 No. 1 p.35 ~ p.38
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Proteome Analysis of Amniotic Fluid by Gradient 2-D PAGE
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Lee Eun-Hee
Kim Jae-Chan
Byeon Sang-Yo
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Abstract
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Analysis of proteome in amniotic fluid was performed by 2-D PAGE (polyacrylamide gel electrophoresis). Proteins in amniotic fluid were separated by centrifugation and solubilized in buffer solution for IEF, using an IPG strip of pH 4-7L. Both a homogeneous slab gel of 12.5% and a gradient gel of 8-18%, were used. After 2-D PAGE, spots were stained with silver nitrate and picked up for in-gel digestion. Digested peptides were analyzed by MALDI-TOF and proteins were further identifical. More protein spots were detected in the gradient gels and a protein not previously reported was identified.
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KEYWORD
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Proteome, amniotic fluid, 2-D PAGE, gradient gel
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